Comixsil RP-AX分析泮庫溴銨與C18的峰形對(duì)比
Column: C18 Leading brand 3u 150*4.6mm
Mobile phase: 乙腈:水:0.25 % H2SO4 =40:10:40
Flow Rate: 1 ml/min
Detection: UV 220 nm
Column: Comixsil RP-AX 3u 150*4.6mm(貨號(hào):FMF-AXR3-EONU)
Mobile phase: 乙腈:水:0.25 % H2SO4 =10:70:20
Flow Rate: 1 ml/min
Detection: UV 220 nm
說明:此應(yīng)用利用 Comixsil RP-AX 的疏水長(zhǎng)鏈保留泮庫溴銨,同時(shí)利用陽離子排斥作用, 改善帶有銨離子分析物的峰形,此類化合物在C18柱上極易形成拖尾峰形。